Prof. Kanamarlapudi Venkateswarlu

Prof. Kanamarlapudi Venkateswarlu

Professor of Molecular Cell Biology and Pharmacology

Swansea University
ILS1, SCHOOL OF MEDICINE, SINGLETON PARK, SWANSEA, United Kingdom, SA2 8PP

plus Speciality

BIOCHEMISTRY

Biography:

I obtained BSc (Biology & Chemistry; 1983-‘86) from Nagarjuna University, MSc (Biochemistry; 1986-’88) from the University of Hyderabad and MTech (Biotechnology; 1988-90) from IIT-Kharagpur.

After working for 3 years as a research scientist at Astra Research Centre India (now AstraZeneca India) in Bangalore on anti-microbial drugs, I was awarded a Commonwealth scholarship to pursue PhD at the University of Sheffield. My PhD training (1993-’96) with Professor Steven Kelly at the University of Sheffield (now Professor at Swansea University) was directed towards understanding azole antifungal drugs mode of action and the mechanisms of azole drug resistance.

After obtaining my PhD degree in 1996, I worked as a postdoctoral researcher for 3 years in Professor Peter Cullen’s laboratory at the University of Bristol on the involvement of lipid second messenger PIP3 in regulation of ADP-ribosylation factor (ARF) small GTPase cell signalling.

I was awarded a prestigious 5 year BBSRC David Phillips Research Fellowship (DPRF) in 1999 to continue my studies (as an independent investigator) on the involvement of PIP3 in regulation of ARF cell signalling in Department of Pharmacology at the University of Bristol. I was appointed as a lecturer in 2004 in the department of Pharmacology at the University of Bristol.

In 2007 I moved to Swansea University to take up Research Reader in Molecular Cellular Biology and Pharmacology position in College of Medicine. I was promoted to a Personal Chair in 2009. I was awarded a DSc by Swansea University in 2016.

Academic positions:

1990 - 1993 Research Scientist, AstraZeneca India, Bangalore, India.

1996 - 1999 Postdoctoral Researcher, Dept. of Biochemistry, Bristol University, UK

1999 - 2004 BBSRC David Phillips Research Fellow, Dept. of Pharmacology, Bristol University, UK

2004 - 2007 Lecturer, Department of Pharmacology, Bristol University, UK

2007 - 2009 Reader in Molecular Cell Biology and Pharmacology, College of Medicine, Swansea University, UK

2009 - present  Professor of Molecular Cell Biology and Pharmacology, College of Medicine, Swansea University, UK

Research interests:

We have been concentrating our efforts for more than 12 years in studying ADP-ribosylation factor (ARF)6 small GTPase and PI 3-kinase mediated cell signalling in health and disease. We have developed novel biochemical and fluorescence based assays to analyse in vivo ARF6 activation and phosphoinositide (PI) binding. We have also isolated ARF6 regulators and their interacting proteins to study spatial assembly of multi-protein complexes. Although it is now established that ARF6 and PI 3-kinase signalling pathways play vital roles in cellular functions, the physiological significance of these signalling pathways has not yet been explored. Therefore, we are currently concentrating our efforts in studying the individual and interplay between ARF6 small GTPase and PI signalling pathways particularly in hematopoietic cells, stem cell differentiation and cancer spreading using a wide variety of molecular, cell biological, biochemical and pharmacological approaches.

Any other information:

Professional Memberships

2004-present   Editorial Board Advisory Panel Member for Biochemical Journal.

2007-present   Biochemical Society UK ‘Cell Biology’ theme panel member

2007-present   NISCHR PhD Grants Panel Member

1993-present   Biochemical Society UK member.

2004-present   British Pharmacological Society member.

2007-present   British Cell Biological Society member

2007-2010       MRC UK College of Experts member

2011-present   Fellow of the Society of Biology (FSB) UK

2011-present   Editorial Board Member for J. Virol. & Mol. Biol.

2012-present   NISCHR Research Grants Panel Member

2012-present   ASBMB member

2012-present   WebMedCentral Cellular Biology Lead faculty member

2012-present   WebMedCentral Plus Speciality editor

2014-present   BBSRC Research Committee D core member

2014-present   Fellow of Royal Society of Chemistry (FRSC) UK

2015-present   Fellow of Association of Biotechnology and Pharmacy (FABAP)

2-15-present    Association of Biotechnology and Pharmacy (ABAP) Senior Scientist Award

 

* = corresponding author

 

  1. Konstantinou D, M. Lei, Z. Xia and V. Kanamarlapudi.*  (2015)  Growth factors mediated differentiation of mesenchymal stem cells to cardiac polymicrotissue using hanging drop and bioreactor.  Cell Biol. Intl. doi: 10.1002/cbin.10409.
  2. Thompson A. and V. Kanamarlapudi.*  (2015)  Agonist-induced internalisation of the glucagon-like peptide-1 receptor is mediated by the Gαq pathway.  Biochem. Pharmacol. 93:72-84.
  3. Thompson A and V. Kanamarlapudi.*  (2014)  The regions within the N-terminus critical for human glucagon like peptide-1 receptor (hGLP-1R) cell Surface expression.  Sci. Rep. 4:7410
  4. Davies J. C., S. Tamaddon-Jahromi, R. Jannoo and V. Kanamarlapudi.*  (2014)  Cytohesin 2/ARF6 regulates preadipocyte migration through ERK1/2 activation.  Biochemical Pharmacol. 92:651-660.
  5. Kanamarlapudi V.*  (2014)  Exchange factor EFA6R requires C-terminal targeting to the plasma membrane to promote cytoskeletal rearrangement through the action of ARF6.  J. Biol. Chem. 289:33378-33390.
  6. Francis W. R., S. E. Owens, C. Wilde, I. Pallister, V. Kanamarlapudi, W. Zou and Z. Xia.  (2014)  The temporal expression of estrogen receptor alpha-36 and runx2 in human bone marrow derived mesenchymal stromal cells during osteogenesis.  Biochem Biophys Res Commun. 453:552-556.
  7. Chan C. H., I. L. Pieper, S. Fleming, Y. Friedmann, G. Foster, K. Hawkins, C. A. Thornton and V. Kanamarlapudi.  (2014)  The effect of shear stress on the size, structure, and function of human von Willebrand factor.  Artif Organs. 38:741-50.
  8. Davies J. C., S. C. Bain and V. Kanamarlapudi.*  (2014)  ARF6 regulates endothelin-1 induced lipolysis in adipocytes.  Biochem. Pharmacol. 90: 406-413.
  9. Thompson A. and V. Kanamarlapudi.*  (2013) Type 2 Diabetes Mellitus and Glucagon Like Peptide-1 Receptor Signalling.  Clin. Exp. Pharmacol. 3: 138. doi:10.4172/2161-1459.1000138
  10. Tu Y, Xue H, W. Francis, A. P. Davies, I. Pallister, V. Kanamarlapudi, and Z. Xia.  (2013)  Biochem. Biophys. Res. Commun. 441: 249-55.
  11. Kanamarlapudi, V.,* S. E. Owens, K. Saha, R. J. Pope and S. J. Mundell.  (2012)  ARF6-dependent regulation of P2Y receptor traffic and function in human platelets.  PLoS ONE 7: e43532.
  12. Eva, R., S. Crisp, J. Marland, J. Norman, V. Kanamarlapudi, C. fFrench-Constant, and J. Fawcett.  (2012)  ARF6 Directs Axon Transport and Traffic of Integrins, and Regulates Axon Growth in adult DRG neurons.  J. Neuro. Sci. 32:10352-10364.
  13. Kanamarlapudi, V.,* A. Thompson, E. Kelly and A. Lopez Bernal.  (2012)  ARF6 activated by the LHCG receptor through the cytohesin family of guanine nucleotide exchange factors mediates the receptor internalisation and signalling.  J. Biol. Chem. 287: 20443–20455.
  14. Kanamarlapudi, V.,* S. E. Owens, J. Lartey and A. Lopez Bernal.  (2012)  ARF6 expression and activities are reduced in myometrium in complicated pregnancies.  PLoS ONE 7: e37954.
  15. Gosney B. J. and V. Kanamarlapudi.* ADAP2. UCSD-Nature Molecule Pages (2011). (doi:10.1038/mp.a000615.01).
  16. Thompson A. and V. Kanamarlapudi.* Arf-like protein 8A. UCSD-Nature Molecule Pages (2011). (doi:10.1038/mp.a003891.01).
  17. Davies J. C. and V. Kanamarlapudi.* SMAP2. UCSD-Nature Molecule Pages (2011). (doi:10.6072/H0.MP.A004105.01)
  18. Thompson A. and V. Kanamarlapudi.* Arf-like protein 8B. UCSD-Nature Molecule Pages (2011). (doi:10.1038/mp.a003892.01).
  19. Kanamarlapudi V.* and Lisa-Marie Wilson. ADP-ribosylation factor domain protein 1. UCSD-Nature Molecule Pages (2011). (doi:10.1038/mp.a003292.01).
  20. Davies J. C. and V. Kanamarlapudi.* SMAP1. UCSD-Nature Molecule Pages (2010). (doi:10.1038/mp.a003290.01).
  21. Owens S. E. and V. Kanamarlapudi.* AGAP1. UCSD-Nature Molecule Pages (2010). (doi:10.1038/mp.a000225.01).
  22. Owens S. E. and V. Kanamarlapudi.* ARAP3. UCSD-Nature Molecule Pages (2010). (doi:10.1038/mp.a003288.01).
  23. Gosney B. J. and V. Kanamarlapudi.* ACAP1. UCSD-Nature Molecule Pages (2010). (doi:10.1038/mp.a000164.01).
  24. Gosney B. J. and V. Kanamarlapudi.* ADAP1. UCSD-Nature Molecule Pages (2010). (doi:10.1038/mp.a000614.01).
  25. Kalscheuer, V. M., L. Musante, C. Fang, K. Hoffmann, C. Fuchs, E. Carta, E. Deas, K. Venkateswarlu, et al.  (2009)  A Balanced Chromosomal Translocation Disrupting ARHGEF9 Is Associated With Epilepsy, Anxiety, Aggression, and Mental Retardation.  Human Mutat. 30: 61-68.
  26. Steward, R., L. Song, S. M. Carter, C. Sigalas, N. R.. Zaccai, V. Kanamarlapudi, M. B. Bhat, H. Takeshima and R. Sitsapesan (2008)  Single-Channel Characterization of the Rabbit Recombinant RyR2 Reveals a Novel Inactivation Property of Physiological Concentrations of ATP.  J. Memb. Biol. 222:65-77.
  27. Bouschet, T., S. Martin, K. Venkateswarlu, S. Mundell and J. M. Henley.  (2007)  The calcium sensing receptor remodels cell shape via β-arrestin-ARNO-ARF6-ELMO protein network.  J. Cell Sci. 120:2489-2497.
  28. Venkateswarlu, K.,* K. G. Brandom and H. Yun.  (2007)  PI 3-kinase-dependent membrane recruitment of centaurin-a2 is essential for its effect on ADP-ribosylation factor 6-mediated actin cytoskeleton reorganisation.  J. Cell Sci. 120:792-801.
  29. Kanumilli, S., E. W. Tringham, C. E. Payne, J. R. B. Dupere, K. Venkateswarlu and M. M. Usowicz.  (2005)  Alternative splicing generates a smaller assortment of CaV2.1 transcripts in cerebellar Purkinje cells than in the cerebellum.  Physiol. Genomics 24: 86-96.
  30. Venkateswarlu, K.*  (2005)  Centaurin-a1 and KIF13B kinesin motor protein interaction in ARF6 signalling. Biochem. Soc. Trans. 33:1279-1281.
  31. Gunn-Moore, F. J., G. I. Welsh, L. R. Herron, F. Brannigan, K. Venkateswarlu,.et al.  (2005)  A novel 4.1 ezrin radixin moesin (FERM) containing protein, ‘Willin’.  FEBS Letts. 579: 5089-5094.
  32. Venkateswarlu, K.*  (2005)  Assay and functional properties of cytohesin interactor IPCEF1.  Meth. Enzymol. 404: 252-266.
  33. Venkateswarlu, K.(2005)  Centaurin-a1 interacts directly with motor protein KIF13B.  J. Cell Sci., 118: 2471-2484.
  34. Lawrence, J. L., S. J. Mundell, H. Yun, E. Kelly and K. Venkateswarlu.*  (2005).  Centaurin-a1, an ARF6 GTPase activating protein, inhibits internalization of b2-Adrenoceptor.  Mol. Pharm. 67: 1821-1827.
  35. Venkateswarlu, K.,* K. G. Brandom and J. L. Lawrence.  (2004)  Centaurin-a1 is an in vivo PIP3-dependent GAP for ARF6 that is involved in actin cytoskeleton organization.  J. Biol. Chem., 279: 6205-6208.
  36. Venkateswarlu, K.*  (2003)  IPCEF1 interacts with and regulates cytohesin 2 activity.  J. Biol. Chem. 278: 43460-43469.
  37. Kanumilli, S., N. J. Toms, K. Venkateswarlu, H. Mellor and P. J. Roberts (2002).  Functional coupling of rat mGluR1a receptor to phospholipase D in CHO cells: Involvement of extracellular Ca2+, PKC, tyrosine kinase and RhoA.  Neuropharm., 42:1-8.
  38. Bobe,R., J. Wilde, P. Maschberger, K. Venkateswarlu, P. J. Cullen, W. Siess and S. P Watson.  (2001)  PI 3-kinase dependent translocation of phospholipase Cg2 in mouse megakaryocytes is independent of Btk translocation.  Blood, 97: 678-684.
  39. Dubios, T., P. Kerai, E. Zemlickova, S. Howell, T. R. Jackson, K. Venkateswarlu, A. B. Theibert, L.Larose, P. J. Roach and A. Aitken.  (2001)  Casein kinase I associates with members of the centaurin- family of phosphatidylinositol-(3,4,5)-trisphosphate-binding proteins.  J. Biol. Chem., 276: 18757-18764.
  40. Lamb, D.C., A. G. S. Warrilow, K. Venkateswarlu, D. E. Kelly and S. L. Kelly.  (2001)  Activities and kinetic mechanisms of native and soluble NADPH-cytochrome P450 reductase.  Biochem. Biophys. Res. Commun., 286: 48-54.
  41. Lamb, D. C., N. N. Kaderbhai, K. Venkateswarlu, D. E. Kelly, S. Kelly and M. A. Kaderbhai.  (2001)  Human sterol 14a-demethylase activity is enhanced by the membrane-bound state of cytochrome b5.  Arch. Biochem. Biophys., 395: 78-84.
  42. Venkateswarlu, K. and P. C. Cullen.  (2000)  Signalling via ARF6 lies downstream of PI 3-kinase.  Biochem. J., 345: 719-724.

 

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